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Gori julian. The mesothelium is composed of an extensive monolayer of specialized cells mesothelial cells that line the bodys serous cavities and internal organs. Ground of reactive mesothelial cells. The article deals with cytopathology specimens from spaces lined with mesothelium ie.
Reactive mesothelial cells may sometimes be sampled in mediastinal fine needle aspirates and should not be mistaken as thymoma cells. It deals with pericardial fluid peritoneal fluid and pleural fluid. Notice in the image on the right how indistinct the cytoplasmic borders are in this clump compared to normal mesothelial cells.
The mesothelial cells in this fluid resemble those seen in many non neoplastic effusions and essentially lack cytologic criteria of malignancy despite this fluid being from a dog with documented mesothelioma based on tissue invasion in surgical biopsies of tissues. Mesothelial cells form a monolayer of specialised pavement like cells that line the bodys serous cavities and internal organs. Mesothelial cytopathology is a large part of cytopathology.
Reactive mesothelial cells tend to come in clusters and clumps and have a more washed out cytoplasm in body fluids. Fetsch aylin simsir andrea abati comparison of antibodies to hbme1 and calretinin for the detection of mesothelial cells in effusion cytology diagnostic cytopathology 101002dc2028 25 3 158 161 2001. Release of protein 90k was significantly increased by treatment with ifn gamma in both mesothelial and ovarian cancer cells.
The wide separation of the nuclei and the well defined nucleoli help to identify these as reactive. However mesothelial cells play other pivotal rol. The cells with dense cytoplasm were identied when the nucleus and cytoplasm stained with about equal inten sity8 despite this feature is characteristic for mesothelial origin it was identied both in mesothelioma 75 reac tive mesothelial proliferation 666 and in adenocarci noma cases 55.
Peritoneal mesothelium was found to produce five fold more 90k than ovarian cancer cells. They contain ovoid nuclei fine chromatin delicate nuclear membrane small nucleoli and a moderate.
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