Simmons Citrate Agar Test Results, Lab Quiz 3 Flashcards Quizlet

Citrate agar is used to test an organisms ability to utilize citrate as a source of energy.

Littlest pet shop coloring pages printable free. Simmons citrate agar is a selective and differential medium that tests for an organisms ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. Citrate utilization can be used to distinguish between coliforms such as klebsiella formerly enterobacter aerogenes ve which occur naturally in the soil and. The citrate utilization test is a part of the imvic test indole methyl red vogues proskauer and citrate test that differentiates organisms on the basis of their ability to use citrate as a sole source of energythe citrate test is performed along with the other imvic tests to differentiate gram negative bacilli of the enterobacteriaceae family.

This medium consists of inorganic salts in which an ammonium salt was the only nitrogen source and citrate being the only carbon source to differentiate between escherichia coli and enterobacter aerogenes. Incubate at 350c to 370c for 18 to 24 hours. The simmons citrate agar test is used for gram negative bacteria on the basis of citrate utilization.

Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Some organisms may require up to 7 days of incubation due to their limited rate of growth on citrate medium. Simmons citrate agar contains sodium citrate as the sole source of carbon ammonium dihydrogen phosphate as the sole source of nitrogen other nutrients and the ph indicator bromthymol blue.

Understand the underlying mechanism of the citrate utilization test. Learning objectives upon completion of this exercise a student should be able to. A commercially available simmons citrate agar medium hardy diagnostics will be used for this purpose.

Inoculate simmons citrate agar lightly on the slant by touching the tip of a needle to a colony that is 18 to 24 hours old. This test is among a suite of imvic tests indole methyl red vogues proskauer and citrate that are used to differentiate among the gram negative bacilli in the family enterobacteriaceae. Simmons citrate test is used for the detection of carbon utilization bacteria from simmons citrate agar.

Simmons citrate agar test. Simmons citrate agar is an agar medium used for the differentiation of enterobacteriaceae based on the utilization of citrate as the sole source of carbon. Citrate utilization test is commonly employed as part of a group of tests the imvic indole methyl red vp and citrate tests that distinguish between members of the enterobacteriaceae family based on their metabolic by products.

It is used for differentiating gram negative bacteria on the basis of citrate utilization. In this method a test microorganism first inoculated with the simmons citrate agar which is composed of citrate ammonium few inorganic ion for growth and a ph indicator such as bromothymol blue. Principle of citrate utilization test.