Pleural Fluid Malignant Mesothelial Cells, The Value Of Cytology And Pleural Biopsy In The Differential Diagnostic Of Nonspecific Pleural Effusions

The distinction between reactive and malignant mesothelial cells has long been a challenge in effusion cytology.

What is mesothelioma definition. Paraffin embedded cell blocks of serous effusions from 32 patients 11 metastatic adenocarcinomas 16 reactive mesothelial proliferations and 5 malignant mesotheliomas were immunostained with ber ep4. Sixty percent of mpe will have positive cytology although this figure is lower in mesothelioma. 2 3 however establishing the diagnosis of mpe can be challenging based on cytomorphologic features alone on routinely stained slides.

Of 16 cases of rm the direct causes of the effusions were infectious in 9 cases combined infectious and malignancy lung involvement in 3 cases malignant with lung involvement in 1 case traumatic in 1 case and unknown in 2 cases. Abouzgheib w bartter t dagher h pratter m klump w. A prospective study of the volume of pleural fluid required for accurate diagnosis of malignant pleural effusion.

Ada is an enzyme that plays an important role in lymphoid cell differentiation. We demonstrated that pleural fluid post lung surgery is a source of mesothelial cells. The observed peak of mesothelial cells on pod3 is consistent with a potential reparative role of free floating mesothelial cells after pulmonary.

In biopsy specimens pleural invasion aids in the diagnosis of malignant mesothelioma. Cytologic evaluation of pleural effusion plays a key role in establishing the diagnosis of malignant pleural effusion mpe and providing material for ancillary tests such as molecular studies and assessment of biomarkers predictive of response to targeted therapies. In contrast in inflammatory conditions the mesothelial lining was usually replaced by granulation tissue although sheets or clumps of exfoliated mesothelial cells were often present in the corresponding pleural fluid clot.

This approach reduces the sensitivity of the method to approximately 60. Cytologylymphocyte subsets pleural fluid cytology a quick and easy method of obtaining material for analysis can potentially confirm the diagnosis of malignant pleural effusion by identification of malignant cells. To evaluate the efficacy of ber ep4 in distinguishing epithelial cells from mesothelial cells in routine cytologic preparations.

However in cytologic specimens in which invasion per se cannot be assessed malignant mesothelioma is difficult to diagnose with certainty. Most of these cells appear to be viable and as shown by cd71 staining activated mesothelial cells. Some multilayering of parietal mesothelium was occasionally seen in chronic pleurisy and around metastases.