Eml4 Alk Fusion Protein, Http Eprints Whiterose Ac Uk 112577 1 Mdw693 Pdf

Fusion protein eml4 alk the inversion within chromosome 2p leads to formation of a fusion product of 4 kb.

Christmas mindful colouring free. The echinoderm microtubule associated protein like 4 anaplastic lymphoma kinase eml4 alk fusion gene resulting from an inversion within chromosome 2p occurs in approximately 5 of non small cell lung cancer and is mutually exclusive with ras and egfr mutations. The crystal structure of a conserved tubulin binding region of the eml1 protein reveals a highly atypical fold in one of its b propeller domains. The eml4 alk fusion gene is responsible for approximately 3 5 of non small cell lung cancer nsclc.

Tumors featuring eml4 alk fusion constitute one subtype of nsclc that might be highly sensitive to alk inhibitors. Tumors in nude mice it has remained unclear whether this fusion protein plays an essential role in the. The vast majority of cases are adenocarcinomas.

Eml4 alk positive lung cancer is a primary malignant lung tumor whose cells contain a characteristic abnormal configuration of dna wherein the echinoderm microtubule associated protein like 4 gene is fused to the anaplastic lymphoma kinase alk genethis abnormal gene fusion leads to the production of a protein that appears in many cases to promote and maintain the malignant behavior of the. The echinoderm microtubule associated protein like 4 eml4 anaplastic lymphoma kinase alk fusion gene was identified in patients with non small cell lung cancer. Eml4 alk is a fusion type protein tyrosine kinase that is generated in human non small cell lung cancer nsclc as a result of a recurrent chromosome inversion inv 21p21p23.

Very recently we have found a novel fusion product between the echinoderm microtubule associated protein like4 eml4 and the anaplastic lymphoma kinase alk in non small cell lung cancers nsclcs. To the best of our knowledge there are only three cell lines harboring the eml4 alk fusion gene which have contributed to the development of therapeutic strategies. The vast majority of cases are adenocarcinomas.

Nucleotide sequencing of this genomic fragment revealed that intron 13 of eml4 is distrupted at a point 36 kb downstream of exon 13 and is inverted to connect to a position 297 bp upstream of exon 21 of alk yielding eml4 alk product. Eml4 alk is a fusion type protein tyrosine kinase that is present in 4 to 5 of cases of nonsmall cell lung cancer and is generated as a result of a small inversion within the short arm of. Although mouse 3t3 fibroblasts expressing human eml4 alk form transformed foci in culture and sc.

To date at least 15 eml4 alk variants have been identified with some. Disruption of the eml1 core region domain in many of the oncogenic eml4alk fusion protein variants that drive nonsmall cell lung cancer explains their dependence on the hsp90 molecular chaperone provides a basis to allow more precise. The standard test used to detect this gene in tumor samples is fluorescence in situ hybridization fish by a us fda approved kit.