Desmin Staining In Mesothelioma, Http Www Britishcytology Org Uk Resources Serous Fluids And Washings Morphological And Immunohistochemical Patterns In Reactive And Neoplastic Conditions Pdf

3 5 8 9 20 the exact etiology for expression of desmin in mesothelial cells is not known.

Lawrence lawry. Staining generally strong and diffuse. Desmin when initially identified in 1977 this intermediate filament was given the designation skeletin. However the multipotential role of mesothelial cells with possible muscle differentiation and coexpression of desmin have been proposed by some.

Desmin and neural marker expression in mesothelial cells and mesotheliomas j. It consists of a headpiece a tailpiece and an intervening alpha helical midpiece. Hurlimann md the distribution of keratin vimentin desmin muscular actin 5100 specific neuron enolase and chromogranin was studied by immunoperoxidase staining in mesothelium malignant mesotheliomas and pulmonary carcinomas.

The muscle marker desmin was useful in differentiating benign from malignant effusions but not in distinguishing mesotheliomas from adenocarcinomas. Conversely a combination of negative ema and positive desmin favors a reactive process. Common stain used in panels for mesenchymal markers confirms myogenic origin of tissue tumors helps differentiate smooth muscle tumors desmin from gist c kit almost always desmin in serous effusion more common in reactive mesothelial cells 84 than mesothelioma 8 or carcinoma 2 am j surg pathol 2001251405.

Ema negativity and desmin positivity were found in 86 55 of 64 of reactive mh cases and 0 of mm cases. Moc 31 was both highly sensitive and specific for detecting adenocarcinoma and was useful as part of a panel of stains in differentiating cells of mesothelial origin from adenocarcinoma. The intermediate filament protein desmin is a known marker for smooth and skeletal muscle differentiation.

The results for desmin and a sma are shown in table 4. The latter is highly conserved between species. 2 810 3 3440.

In 26 cases desmin stained more than 75 of cells with high intensity in 5 cases 50 of cells with moderate intensity and in 3 cases less than 25 of cells with low intensity. It has a molecular weight of 53 kd. In addition 60 of sarcomatoid mesotheliomas were positive for asma and the positive reactions for asma were wider and stronger than those for desmin.

In addition 60 of sarcomatoid mesotheliomas were positive for a sma and the positive reactions for a sma were wider and stronger than those for. Likewise strong membranous positivity for glut 1 andor strong nuclear staining for p53 favors a mesothelioma. In sarcomatoid mesothelioma positive staining for desmin was observed only in three cases 8 but the reaction was focal 1 or 2 and weak.